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KMID : 1101420170490010001
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Korean Journal of Clinical Laboratory Science
2017 Volume.49 No. 1 p.1 ~ p.7
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Enhancing the Effects of Zerumbone on THP-1 Cell Activation
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Lee Min-Ho
Kim Sa-Hyun
Yu Sung-Ryul
Lee Pyeong-Jae
Moon Cheol
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Abstract
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Zerumbone is a major component of the essential oil from Zingiber zerumbet Smith, which is a kind of wild ginger. In addition, various biological functions, such as liver protection, pain relief, atherosclerosis, and antimicrobial activity have been reported. It is also known to be effective in the proliferation of immune cells and the expression of cytokines. In this study, we investigated the effects of zerumbone on monocyte activation. First, it was confirmed that the proliferation of THP-1 cells was increased by zerumbone. The strongest increase in THP-1 proliferation after lipopolysaccharide treatment was observed at 5 μM zerumbone treatment, and the increase of cell proliferation without lipopolysaccharide was the highest at 10 μM. Conversely, when treated with 50 μM zerumbone, a rapid decrease of proliferation was observed regardless of the presence of lipopolysaccharide (LPS). The phosphorylation of signaling protein, Erk, induced by LPS was also increased by zerumbone. The strongest increase in phosphorylation was observed when treated with 50 μM of zerumbone with reduced proliferation. The activity of transcription factor NF-κB was not significantly altered by zerumbone alone, but increased when treated with lipopolysaccharide. Furthermore, the transcription of the inflammatory cytokines TNF-α and IL-8, which are regulated by NF-κB, is also increased by zerumbone. These results suggest that zerumbone can enhance the proliferation and activity of monocytes. Furthermore, it is believed that zerumbone can enhance rthe immune responses through increased monocyte activity in bacterial infections with LPS, thereby helping to treat effective bacteria.
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KEYWORD
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Zerumbone, Monocyte, Lipopolysaccharide
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